Rapid sampling of yeast cells and automated assays of adenylate, citrate, pyruvate and glucose-6-phosphate pools |
| |
| Authors: | K E Weibel J R Mor A Fiechter |
| |
| Affiliation: | Institute of Microbiology, Swiss Federal Institute of Technology (ETHZ), Zürich, Switzerland |
| |
| Abstract: | A sampling technique for yeast cells with a very short interval between harvesting and inactivation of the cells is described. HClO4 serves as inactivating agent. Optimal extraction is achieved by three freeze-thaw cycles of the acidic solution followed by neutralization with 2 m KHCO3 to pH 7.5. Methods are presented for the automated fluorometric analyses of ATP, ADP, AMP, glucose-6-phosphate, pyruvate and citrate, as well as for the preparation of crude cell extracts. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
