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| 同步辐射软X射线对枯草杆菌的诱变效应 | |
| 引用本文： | 温崇庆,唐欣昀,蒋诗平,徐至展,孙亦阳.同步辐射软X射线对枯草杆菌的诱变效应[J].激光生物学报,2004,13(1):30-33. |
| 作者姓名： | 温崇庆 唐欣昀 蒋诗平 徐至展 孙亦阳 |
| 作者单位： | 1. 安徽农业大学生命科学学院,中国安徽,合肥,230036 2. 中国科学技术大学国家同步辐射实验室,中国安徽,合肥,230029;中国科学院上海光学精密机械研究所,中国,上海,201800 3. 中国科学院上海光学精密机械研究所,中国,上海,201800 |
| 基金项目： | 本研究得到合肥国家同步辐射实验室的资助. |
| 摘 要： | 采用同步辐射软X射线对枯草芽孢杆菌(Bacillus subtilis)1831菌株进行辐照处理,研究了不同剂量下3．1nm的软X射线对其芽孢的失活和诱变作用。结果表明：同步辐射软X射线对枯草杆菌芽孢的剂量存活曲线表现为典型的“肩型”,对芽孢的失活作用属于“单靶多击”方式,失活击中数等于3。根据脱脂牛奶平板上蛋白酶活力大小的测量统计,以变异系数作为诱变效应指标,软X射线对芽孢具有一定的诱变作用。 |
| 关 键 词： | 同步辐射软X射线 枯草芽孢杆菌 失活作用 诱变效应 |
| 文章编号： | 1007-7146(2004)01-0030-04 |
| 收稿时间： | 2003/8/30 |
| 修稿时间： | 2003年8月30日 |
Mutagenic Effect of Soft X-ray of Synchrotron Radiation on Bacillus subtilis |
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| WEN Chong-qing,TANG Xin-yun.Mutagenic Effect of Soft X-ray of Synchrotron Radiation on Bacillus subtilis[J].ACTA Laser Biology Sinica,2004,13(1):30-33. | |
| Authors: | WEN Chong-qing TANG Xin-yun |
| Institution: | WEN Chong-qing~1,TANG Xin-yun~ |
| Abstract: | Bacillus subtilis 1831 strain was treated by soft X-ray of synchrotron radiation. Inactivation and mutagenesis pattern of soft X-ray of synchrotron radiation on B. subtilis 1831 spores were studied. The results showed that the survival curve of spores, which irradiated by 3.1 nm soft X-ray, appeared typical "Shoulder shape", and the inactivation of spores belonged in "Single target multi-hit model", with the number of inactivation hits being 3. Protease activity of colonies on skim milk agar plate was measured and analyzed with statistical method, and the coefficient of variation of the protease activity was expressed as index of mutagenic effect, soft X-ray had certain mutagenesis on spores. |
| Keywords: | soft X-ray of synchrotron radiation Bacillus subtilis inactivation mutagenic effect |
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