| 香蕉束顶病植原体16S rDNA片段的RFLP和序列分析 |
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| 引用本文： | 李横虹,邱并生,李锂,史春霖.香蕉束顶病植原体16S rDNA片段的RFLP和序列分析[J].微生物学报,1999,39(4):315-320. |
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| 作者姓名： | 李横虹 邱并生 李锂 史春霖 |
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| 作者单位： | 中国科学院微生物研究所 |
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| 摘 要： | 香蕉束顶病（ＢＢＴ）是一种发生在蕉类作物的严重病害。从带有典型香蕉束顶病症状的香蕉植株中按照检测植原体的方法提取ＤＮＡ，扩增患病植株中植原体１６ＳｒＤＮＡ片段，证明香蕉束顶病中有植原体存在。对此扩增片段进行限制性酶切片段长度多态性（ＲＦＬＰ）分析和核酸序列分析，并与已知植原体的序列进行同源性比较，构建进化树。结果显示该片段与Ｇｒ１的亲缘关系最近。
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| 关 键 词： | 香蕉束顶病（BBT），植原体，16S rDNA，RFLP，序列分析 |
AMPLIFICATION OF PHYTOPLASMA 16S rDNA FROM BANANA BUNCHY TOP DISEASE,RFLP AND SEQUENCE ANALYSIS OF 16S rDNA FRAGMENT * |
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| Li Henghong,Qiu Bingsheng,Li Li,Shi Chunlin.AMPLIFICATION OF PHYTOPLASMA 16S rDNA FROM BANANA BUNCHY TOP DISEASE,RFLP AND SEQUENCE ANALYSIS OF 16S rDNA FRAGMENT *[J].Acta Microbiologica Sinica,1999,39(4):315-320. |
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| Authors: | Li Henghong Qiu Bingsheng Li Li Shi Chunlin |
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| Institution: | Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080. |
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| Abstract: | Extract DNA from banana plants with banana bunchy top disease and amplify the phytoplasma 16S rDNA fragment by nested PCR. The classification of the phytoplasma established on the basis of 16S rDNA restriction patterns was examined by performing a sequence analysis of 16S rDNA fragment. The RFLP pattern, sequence data and phylogenic tree showed that this phytoplasma should belong to Group I(Phytoplasma asteri). |
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| Keywords: | Banana bunchy top Phytoplasma 16S rDNA RFLP Sequenc e analysis |
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