拟除虫菊酯对家蝇Na-K-ATPase抑制作用的研究

通过对家蝇神经系统Na-K-ATPase性质的研究，表明Na-K-ATPase反应的适宜pH值为7.0～8.0，适温为35～40℃，K_m为0.22 mmol/L，V_max为555.56 nmol/(mg·min)。比较测定了家蝇敏感品系、Del-R、2Cl-R抗性品系的Na-K-ATPase活性及溴氰菊酯和氯菊酯对该酶的抑制作用。实验证明，敏感与抗性品系间Na-K-ATPase活力无显著的差异，但溴氰菊酯和氯菊酯对不同家蝇品系Na-K-ATPase的抑制作用有明显区别，两种拟除虫菊酯可抑制敏感家蝇品系Na-K-ATPase的活性，而对抗性品系无明显的抑制作用。

INHIBITION OF PYRETHROID INSECTICIDES ON NERVE NA-K-ATPASE IN HOUSE FLIES (MUSCA DOMESTICA)

The properties of Na-K-ATPase in the nerve system of house flies were described For-Na-K ATPase the optimum pH and temperature were 7.0～8.0and 35～40℃ respectively, the K_m value was 0.22 mmol/L ATP and Vmax was approximately 555.56 nmol P_i/(mg·protein·min). The Na-K-ATPase activities in susceptible (SP) and Del-R、2Cl-R house fly strains were determined. The results indicated that there was no significant difference in Na-K-ATPase activities among the three strains. Inhibition of deltamethrin and permethrin on Na-K-ATPase activity was also studied. The results showed that the two pyrethroids inhibited obviously the Na-K-ATPase activity in the susceptible strain, but no effect in the resistant strain