Genome-wide screen identifies signaling pathways that regulate autophagy during
Caenorhabditis elegans development |
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| Authors: | Bin Guo Xinxin Huang Peipei Zhang Linxiang Qi Qianqian Liang Xuebo Zhang Jie Huang Bin Fang Wenru Hou Jinghua Han Hong Zhang |
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| Affiliation: | 1College of Life Sciences, China AgriculturalUniversity, Beijing, China;2State Key Laboratory of Biomacromolecules, Institute ofBiophysics, Chinese Academy of Sciences, Beijing, China;3National Institute of Biological Sciences, Beijing, China |
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| Abstract: | ![]()
The mechanisms that coordinate the regulation of autophagy with developmental signaling duringmulticellular organism development remain largely unknown. Here, we show that impaired function ofribosomal protein RPL-43 causes an accumulation of SQST-1 aggregates in the larval intestine, whichare removed upon autophagy induction. Using this model to screen for autophagy regulators, weidentify 139 genes that promote autophagy activity upon inactivation. Various signaling pathways,including Sma/Mab TGF-β signaling, lin-35/Rb signaling, the XBP-1-mediatedER stress response, and the ATFS-1-mediated mitochondrial stress response, regulate the expressionof autophagy genes independently of the TFEB homolog HLH-30. Our study thus provides a framework forunderstanding the role of signaling pathways in regulating autophagy under physiologicalconditions.Subject Categories: Autophagy & Cell Death; Signal Transduction; Membrane & Intracellular Transport |
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| Keywords: | autophagy Caenorhabditis elegans ER stress mitochondrial stress Rb |
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