The effect of temperature on hairy root cultures of Catharanthus roseus: Growth,indole alkaloid accumulation and membrane lipid composition |
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Authors: | Leena Toivonen Simo Laakso Heikki Rosenqvist |
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Institution: | (1) Department of Chemical Engineering, Laboratory of Biochemistry and Microbiology, Helsinki University of Technology, Kemistintie 1, SF-02150 Espoo, Finland |
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Abstract: | Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to an increased proportion of linolenic acid in the main lipid classes. The modifications in lipid composition were obviously necessary for the roots to retain the proper cell membrane fluidity at each temperature. Despite of changes in membrane lipids, no effect on the distribution of indole alkaloids between the roots and the medium could be detected. Instead, the level of alkaloid accumulation showed a clear increase with lowering temperature.Abbreviations PC
phosphatidylcholine
- PE
phosphatidylethanolamine
- PI
phosphatidylinositol
- PS
phosphatidylserine
- PG
phosphatidylglycerol
- CL
cardiolipin
- DGD
digalactosyldiglyceride
- MGD
monogalactosyldiglyceride
- NL
neutral lipids
- DU
degree of fatty acid unsaturation |
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Keywords: | |
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