Interactions of Meso-tetra-(4-N-oxyethylpyridyl) Porphyrin,Its 3-N Analog and Their Metallocomplexes with Duplex DNA |
| |
| Authors: | Yeva B Dalyan Samvel G Haroutiunian Gayane V Ananyan Volodya I Vardanyan Dmitri Y Lando Valeri N Madakyan |
| |
| Institution: | 1. Department of Physics , Yerevan State University , 1, Al.Manoogian St., 375025 , Yerevan , Armenia;2. Institute of Bioorganic Chemistry, Belarus National Academy of Sciences , Kuprevich St. 5/2, 220141 , Minsk , Belarus;3. Yerevan State Medical University , 2, Korun St., 375025 , Yerevan , Armenia |
| |
| Abstract: | Abstract Interactions of meso-tetra-(4-N-oxyethylpyridyl) porphyrin (TOEPyP(4)), its 3-N analog (TOEPyP(3)) and their Co, Cu, Ni, Zn metallocomplexes with duplex DNA have been investigated by uv/visible absorbance and circular dichrosim spectroscopies. Results reveal the interactions of these complexes with duplex DNA are of two types. (1) External binding of duplex DNA by metalloporphyrins containing Zn and Co, and (2) Binding of duplex DNA both externally and internally (by intercalation) by porphyrins not containing metals, and metalloporphyrins containing Cu and Ni. Results indicate that (4N-oxyethylpyridyl) porphyrins intercalate more preferably in the structure of duplex DNA and have weaker external binding than 3N-porphyrins. |
| |
| Keywords: | B?A-transition in DNA Sequence-specificity of local DNA structure GG/CC and AA/TT steps in DNA Intramolecular interactions in DNA |
|
|
