Flexibility of DNA and RNA upon Binding to Different Metal Cations. An Investigation of the B to A to Z Conformational Transition by Fourier Transform Infrared Spectroscopy |
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| Authors: | T Theophanides H A Tajmir-Riahi |
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| Institution: | Department of Chemistry , Université de Montréal , Montréal , Quebec , Canada , H3C 3V1 |
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| Abstract: | Abstract The interaction of DNA and RNA with Cu(II), Mg(II), Co(NH3)6]3+ Co(NH3)5Cl]2+ chlorides and, cis- and trans-Pt(NH3)2Cl2 (CIS-DDP, trans-DDP) has been studied by Fourier Transform Infrared (FT-IR) spectroscopy and a correlation between metal-base binding and conformational transitions in the sugar pucker has been established. It has been found that RNA did not change from A-form on complexation with metals, whereas DNA exhibited a B to Z transition. The marker bands for the A-form (C′3-endo-anti conformation) were found to be near 810–816 cm?1, while the bands at 825 and 690 cm?1 are marker bands for the B- conformation (C′2-endo, anti), The B to Z (C3′-endo, syn conformation) transition is characterized by the shift of the band at 825 cm?1 to 810–816 cm?1 and the shift of the guanine band at 690 cm?1 to about 600–624 cm?1. |
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