Vibrational Analysis of Phosphorothioate DNA: II. The POS Group in the Model Compound Dimethyl Phosphorothioate [(CH3O)2(POS)]- |
| |
| Authors: | C A Steinke K K Reeves J W Powell S A Lee Y Z Chen T Wyrzykiewicz |
| |
| Institution: | 1. Department of Physics &2. Astronomy , University of Toledo , Toledo , OH , 43606 , USA;3. Department of Physics , Reed College , Portland , OR , 97202 , USA;4. Department of Physics , Reed College , Portland , OR , 97202 , USA;5. Astronomy , University of Toledo , Toledo , OH , 43606 , USA;6. ISIS Pharmaceuticals , 2280 Faraday Avenue, Carlsbad , CA , 92008 , USA |
| |
| Abstract: | Abstract The results of Raman and Infrared (IR) spectroscopic investigations on the vibrational modes of dimethyl phosphorothioate (DMPS) anion, (CH3O)2(POS)]?, are reported. Ab initio calculations of the vibrational modes, the IR and Raman spectra and the interatomic force constants of DMPS were performed. A normal mode calculation was performed and the results were used to calculate the potential energy distribution for the vibrational modes. This analysis shows that in DMPS the P-S stretching mode has a frequency of about 630 cm?1 and an angle bending mode involving the sulfur atom has a frequency of about 440 cm?1. The proposed vibrational mode assignments will serve as marker bands in the conformational studies of phosphorothioate oligonucleotides which play a central role in the novel antisense therapeutic paradigm. |
| |
| Keywords: | β-N-Acetyl-D-glucosaminidase Scylla serrata Dioxane Inactivation Kinetics |
|
|
