High-performance liquid chromatographic identification of beta-lactam antibiotics produced by dermatophytes |
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| Authors: | HM Lappin-Scott ME Rogers MW Adlard G Holt WC Noble |
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| Institution: | School of Biotechnology, Faculty of Engineering and Science, The Polytechnic of Central London, 115 New Cavendish Street, London W1M 8JS and Institute of Dermatology, St. John's Hospital for Diseases of the Skin, Homerton Grove, London E9 6BX |
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| Abstract: | Thirteen of 48 dermatophyte isolates were found by bioassay to produce beta-lactam antibiotics and seven produced other antibiotics. Estimation and detection of specific beta-lactams in culture broths by derivatization and HPLC was only possible following concentration and extraction procedures. Analysis of the concentrated broths demonstrated the production of penicillin X and penicillin G by two Trichophyton mentagrophytes strains and by one Microsporum canis strain; one further T. mentagrophytes strain produced only penicillin X. Additions of the beta-lactam side-chain precursors, phenylacetic acid and phenoxyacetic acid, to fermentation media failed to increase the antibiotic titres. |
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