A plasmid-encoded anion-translocating ATPase |
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Authors: | B P Rosen C M Hsu C E Karkaria P Kaur J B Owolabi L S Tisa |
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Institution: | Department of Biochemistry, Wayne State University, School of Medicine, Detroit, MI 48201. |
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Abstract: | An anion-translocating ATPase has been identified as the product of the arsenical resistance operon of resistance plasmid R773. When expressed in Escherichia coli this ATP-driven oxyanion pump catalyzes extrusion of the oxyanions arsenite, antimonite and arsenate. Maintenance of a low intracellular concentration of oxyanion produces resistance to the toxic agents. The pump is composed of two polypeptides, the products of the arsA and arsB genes. This two-subunit enzyme produces resistance to arsenite and antimonite. A third gene, arsC, expands the substrate specificity to allow for arsenate pumping and resistance. |
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