A radioimmunoassay for lithocholic acid conjugates in human serum and liver tissue |
| |
| Authors: | A. Roda E. Roda D. Festi R. Aldini G. Mazzella C. Sama L. Barbara |
| |
| Affiliation: | 1. Department of Gastroenterology, University of Bologna, Italy;1. Institute of Chemistry G. Ciamician, University of Bologna, Italy |
| |
| Abstract: | A sensitive and specific radioimmunoassay for glycine and taurine conjugates of lithocholic acid (CLCA) has been developed. 3H-glycolithocholic acid (S.A. = 17Ci/mmol) was used as tracer. Separation of free from antibody-bound bile acid was carried out using ammonium sulphate (saturated solution). The antiserum showed high specificity for both glyco and tauro conjugated lithocholate (100% cross reaction) and lithocholic acid (25% cross reaction). The sensitivity of the assay (1 pmole/tube), was adequate for measuring CLCA in peripheral blood and hepatic tissue in man. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
