Host and environmental effects on the infection of maize by Puccinia sorghi. II. Post-penetration development

The numbers of pustules which subsequently developed on maize seedlings incubated at 5, 10, 15, and 20 °C and 100% r.h. were directly related to the temperature and length of incubation. The generation time was 16 days at 10 °C, 10 days at 15 °C, 7 days at 20 °C and 5 days at 25 °C. Increase in urediniospore production was observed with increase in temperature. Increase in the inoculum concentration up to 5 × 10⁴ urediniospores/ml increased the number of resultant uredinia. However, further increases did not increase uredinia-formation.