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O uptake rate measurements as a novel tool to study shear effects on suspended strawberry cells |
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| Authors: | Michael Keßler Hens J.G. ten Hoopen Josef J. Heijnen Shintaro Furusaki |
| Affiliation: | (1) Graduate School of Engineering (Dept. of Chemistry and Biotechnology), University of Tokyo, 7-3-1 Hongo, Bunkyo-ku Tokyo, Japan;(2) Biotechnological Science Delft Leiden, Sector Industrial Plant Biotechnology, Dept. of Biochemical Engineering, Delft University of Technology, Julianalaan 67, Delft, Netherlands |
| Abstract: | Despite the same mean volumetric power input of 19 and 47 W/m 3 , the specific oxygen uptake rate (OUR S ) of strawberry ( Fragaria ananassa) cell suspensions was higher in bioreactors equipped with a Rushton Turbine (4.4 and 6.2 3 10 -5 mol O 2 /kg.s) than with an anchor stirrer (2.5 and 4.6 3 10 -5 mol O 2kg.s). The increase in OUR S was caused by stress-activated respiration and appeared to be correlated with the locally dissipated power input. OUR S was corrected for the increase in surface through aggregate break-up and reached a maximum of 6.0 3 10 -5 mol O 2kg.s when agitating with approximately 200 kW/m 3 locally dissipated power input. |
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