Novel microsatellite markers for Dalechampia scandens (Euphorbiaceae) and closely related taxa: application to studying a species complex |
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| Authors: | Mohsen Falahati‐Anbaran Hans K. Stenøien Geir H. Bolstad Thomas F. Hansen Rocío Pérez‐Barrales W Scott Armbruster Christophe Pélabon |
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| Affiliation: | 1. Department of Plant Science, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Sciences, University of Tehran, Tehran, 14155‐6455, Iran;2. NTNU University Museum, Norwegian University of Science and Technology, Erling Skakkes gate 47A, N‐7491 Trondheim;3. Norwegian Institute for Nature Research (NINA), Centre for Biodiversity Dynamics, Postboks 5685 Sluppen, N‐7485 Trondheim;4. Department of Biology, CEES and Evogene, University of Oslo, Oslo, N‐0316, Norway;5. School of Biological Sciences, King Henry Building, King Henry I Street, University of Portsmouth, Portsmouth, PO1 2DY, UK;6. Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, Alaska 99775, USA;7. Department of Biology, Norwegian University of Science and Technology, H?gskoleringen 5, N‐7491 Trondheim;8. Centre for Biodiversity Dynamics, Department of Biology, Norwegian University of Science and Technology, H?gskoleringen 5, N‐7491 Trondheim, Norway |
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| Abstract: | We developed novel microsatellite markers for D alechampia scandens L. (Euphorbiaceae). The target plants belong to a distinct, but undescribed, species in the D . scandens species complex, characterized by small resin‐producing glands. In total, 110 alleles over 36 novel markers were identified across 39 individuals from three populations. The number of alleles varied from one to seven, with an average of 3.06 ± 0.26 alleles per locus. The developed markers, along with previously developed ones for a large‐glanded D . scandens species, were tested for amplification in 11 additional species of the genus D alechampia. Four markers did not produce any detectable allele in 37 individuals from two populations of the large‐glanded species. Average expected heterozygosity across all small‐ and large‐glanded specific loci was 0.36 and 0.15, for the small and large glanded populations, respectively. Cross‐species amplification showed that 89% of all markers were successfully amplified in at least one of the 11 other D alechampia species. These microsatellite markers may be useful for detecting undescribed species in the D . scandens species complex, and can be used for comparative analyses of genetic structure, mating system and phylogeography of other D alechampia species. |
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| Keywords: | cross‐species amplification cryptic biodiversity Dalechampia Euphorbiaceae microsatellite loci species complex |
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