Development of nuclear microsatellite markers for the threatened wetland plant Geranium soboliferum var. kiusianum (Geraniaceae) |
| |
| Authors: | Seikan Kurata Shota Sakaguchi Motomi Ito |
| |
| Affiliation: | 1. Laboratory of Plant Evolution and Biodiversity, Department of General Systems Studies, Graduate School of Arts and Sciences, The University of Tokyo, 3‐8‐1 Komaba, Meguro‐ku, Tokyo 153‐8902, Japan;2. Faculty of Integrated Human Studies, Graduate School of Human and Environmental Studies, Kyoto University, Yoshida‐nihonmatsu‐cho, Sakyo‐ku, Kyoto 606‐8501, Japan |
| |
| Abstract: | ![]()
Nuclear microsatellite markers were developed for the threatened plant Geranium soboliferum var. kiusianum, which has decreased its population size as a result of loss of its wetland habitat in Kyushu, Japan. Utilizing RNA‐seq data obtained by next‐generation sequencing techniques, 10 polymorphic microsatellite markers with 3–16 alleles in a nuclear genome were developed and characterized. Two to 15 alleles were observed in G. soboliferum. These markers will be used to investigate the genetic circumstance of remnant populations of G. soboliferum var. kiusianum and their phylogenetic relationship with G. soboliferum. |
| |
| Keywords: | genetic diversity
Geranium soboliferum var. kiusianum
microsatellite marker relict plant wetland plant |
|
|
