Production of monoclonal antibodies against Clostridium botulinum type E derivative toxin |
| |
Authors: | Yohichi Kamata Shunji Kozaki Takafumi Nagai Genji Sakaguchi |
| |
Institution: | Department of Veterinary science, College of Agriculture, University of Osaka Prefecture, Sakai-shi, Osaka 591, Japan |
| |
Abstract: | Abstract Five monoclonal antibodies (MCA; E–8–2, 9–1, 11–2, 12–4, and 13–1) against Clostridium botulinum type E derivative toxin were prepared. Their ELISA titers were higher than or equivalent to that of conventional polyclonal antibody. Three of them (E-8–2, 12–4, and 13–1) possessed the neutralizing activity comparable to that of polyclonal antibody. The results of binding-competition experiments indicated that the monoclonal antibodies bound to different sites on the type E toxin molecule. Immunoblotting analyses demonstrated that E-8–2, 9–1, and 11–2 react to fragment I (heavy chain) of the toxin. By use of these monoclonal antibodies, it may be possible to scrutinize the structure-function relationship of botulinum toxins and cross reactions between type E and F toxins. |
| |
Keywords: | Type E botulinum toxin monoclonal antibodies ELISA |
本文献已被 ScienceDirect 等数据库收录! |
|