Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms (PCR-RFLP) and Electrophoretic Assays for the Mouse Obese (Lepob) Mutation

Three polymerase chain reaction-based assays for the mouse Lep^ob mutation are described: Dde I site created by the C←T transversion characterizing Lep^ob enables positive detection of the mutant allele; positive detection of the wild-type Lep allele is achieved by the use of primer sequence which introduces an A←C substitution, creating an Msp I site in the normal allele; and an electrophoretic assay which positively identifies the hetero-zygote.