Double-stranded RNA and virus-like particles in the edible basidiomycete Flammulina velutipes (Enokitake) |
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| Authors: | Magae Y Hayashi N |
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| Institution: | Bio-resources Technology Division, Forestry and Forest Products Research Institute, P.O. Box 16, Tsukuba Norin, Ibaraki, Japan. ymagae@ffpri.affrc.go.jp |
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| Abstract: | One of the commercial strains of Flammulina velutipes was analyzed for the presence of double-stranded RNA (dsRNA) elements to examine the underlying mechanism of strain degeneration. As a result, two dsRNA elements sized 1.9 and 1.8 kb were detected in mycelium derived from spontaneously brown-colored fruit body. They were not detected in the normal strains or in fruiting-impaired degenerative isolates. The dsRNAs were not in the nuclear or mitochondrial fractions, but were located in the cytoplasmic fraction. The presence of virus-like particles of ca. 50 nm diameter associated with the dsRNAs was confirmed by electron microscopic observation. |
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| Keywords: | Double-stranded RNA Virus-like particle Degeneration Flammulina velutipes |
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