Total protein and isozyme characterization in the flax zygotic embryo during development |
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Authors: | A Pretová Bohuˇs Obert Martin Hajduch Edita Gregová |
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Institution: | (1) Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Akademická 2, P.O. Box 39A, 95007 Nitra, Slovak Republic e-mail: pretova@savba.savba.sk Fax: +421-87-73-36660; Tel.: +421-87-73-36659,;(2) Research Institute of Plant Production, Bratislavská cesta 122, SK-92168 Pieˇstany, Slovak Republic, |
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Abstract: | Flax zygotic embryogenesis was studied for isozyme patterns of acid phosphatase and esterase and for content of total proteins.
For acid phosphatase, six multiple molecular forms or isozymes, were identified during flax zygotic embryo development. For
esterase, six isozymes were expressed during zygotic flax embryogenesis. Some of the isozymes were expressed during entire
embryogenesis, and some were only transiently expressed or appeared near maturation. The amount of total proteins was very
low at early embryogenesis and proteins in a range from 26.6 kDa to 97.4 kDa were expressed. During further flax embryo development
the protein spectra became more diverse and the quantity of total proteins increased. Proteins ranging from 3.4 kDa to 97.4
kDa were present, and proteins of 20 kDa to 36.5 kDa were expressed in the highest amount. The results are discussed with
reference to selected information in the literature.
Received: 16 October 2000 / Revision accepted: 20 April 2001 |
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Keywords: | Zygotic embryogenesis Flax Isozymes Esterase Acid phosphatase Total proteins |
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