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The types of bioreactors used for shoots and embryos
Authors:Shinsaku Takayama  Motomu Akita
Institution:(1) Department of Biological Science and Technology, Tokai University, 317 Nishino, 410-03 Numazu, Shizuoka, Japan;(2) Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University, 930 Nishimitani, Uchita, Naga, 649-64 Wakayama, Japan
Abstract:Plant regenerated organs such as shoots, bulbs, microtubers, corms, embryos, etc. have been successfully proliferated in the bioreactor. The use of a bioreactor leads to the development of technology suitable for large scale plant propagation. The basic construction and characteristics of various types of bioreactor systems are reviewed in relation to shoot and embryo cultures. A pilot scale 500 liter bioreactor system was applied to the production of large scale Stevia rebaudiana shoots.Abbreviations DW dry weight - EC electrical conductivity - FW fresh weight - ORP oxidation-reduction potential
Keywords:bioreactor  embryo culture  mass propagation  shoot culture  Stevia rebaudiana  types of bioreators
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