产双羔彭波半细毛羊发情期卵巢基因表达差异研究

彭波半细毛羊是西藏自治区第一个国家级家畜新品种,对其产羔性状的研究有助于推动藏绵羊繁殖力研究工作。为研究产双羔彭波半细毛羊发情期内卵巢基因的特异调控模式,采用转录组测序对其差异表达基因进行了筛选和分析。结果发现,双羔组相对于单羔组有1 021个基因表达上调,1 468个基因表达下调。GO和KEGG分析发现,上调基因主要与细胞膜结构相关,下调基因主要与物质代谢等相关。其中,上调基因显著富集于与细胞膜相关的细胞粘附、细胞运动、物质运输、信号转导等生命活动。研究结果表明,双羔组卵巢内细胞的物质运输和信号转导频率加快,以促进卵泡的分化和发育;同时,双羔组卵巢内细胞的粘附及运动功能增强,有利于卵母细胞的发育和成熟卵子的排出。此外,卵巢中细胞粘附因子、TGF-beta信号通路和溶质运载蛋白家族基因的上调表达可作为彭波半细毛羊产双羔性状的潜在信号进行更深入的研究。研究结果不仅有利于对彭波半细毛羊产双羔性状的研究和利用,更有利于人们对藏绵羊繁育力的深入研究。

Expression Differences of Ovarian Genes of Pengbo Semi Fine Wool Sheep Producing Twin Lambs at Estrus

Pengbo semi fine wool sheep is the first national new breed of sheep in Tibet Autonomous Region,and the study on the litter size can facilitate the development of fecundity research in Tibetan sheep.In this study,RNA-sequencing was applied to investigate the regulation pattern of ovarian gene during the estrus period of Pengbo semi fine wool sheep producing twin lambs(dual group).The results showed that a total of 1021 up-regulated and 1468 down-regulated genes were detected in the dual group compared with the single group.GO and KEGG analysis revealed that the up-regulated genes were mainly related to cell membrane structure(cell adhesion,cell movement,material transport and signal transduction processes),while the down-regulated genes were mainly related to material metabolism.These results indicated that the frequency of material transport and signal transduction in the ovary of dual group contributed to the promotion of differentiation and the development of follicles.Moreover,the enhanced adhesion and motor function of the cells might facilitate the development of oocytes and the discharge of mature ovum.Further study concerning on the up-regulated cell adhesion molecules,TGF-beta signaling pathway and solute carrier family genes in ovary can contribute to developing potential marks for twin-lambs production.Therefore,this study is not only helpful to the litter size research of Pengbo semi fine wool sheep,but also contributes to the development of fecundity research in Tibetan sheep.