| 流加发酵对重组Bacillus subtilis发酵生产角质酶的影响 |
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| 引用本文： | 陈晟,张芙华,陈坚,吴敬.流加发酵对重组Bacillus subtilis发酵生产角质酶的影响[J].中国生物工程杂志,2010,30(1):62-66. |
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| 作者姓名： | 陈晟 张芙华 陈坚 吴敬 |
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| 作者单位： | 1.江南大学生物工程学院 工业生物技术教育部重点实验室 无锡 214122
2.江南大学食品科学与技术国家重点实验室 无锡 214122 |
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| 基金项目： | 国家“863”计划(2009AA02Z204); 教育部新世纪优秀人才支持计划(NCET-06-0486); 食品科学与技术国家重点实验室科研基金(SKLF-MB-200802)资助项目 |
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| 摘 要： | 为实现基因工程菌Bacillus subtilis WSHB06-07生产角质酶的高产，在3L发酵罐中考察了不同初糖浓度对菌体生长和产酶的影响，并在选择38 g/L初始蔗糖浓度的基础上，进行碳源的分批流加和恒速流加，结果表明发酵16 h开始流加碳源，采用总补糖量60g/L，蔗糖平均流速为4g/(L·h)的恒速补料方式，角质酶酶活在31h可达到最大545.87U/ml，比分批发酵酶活提高67.8%，并获得较高的角质酶生产强度，满足工业化生产要求。
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| 关 键 词： | Bacillus subtilis 重组角质酶 分批流加发酵 连续流加发酵 碳源 生产强度 |
| 收稿时间： | 2009-09-01 |
| 修稿时间： | 2009-11-01 |
Effects of Fed-fermentation on Cutinase Production by Recombinant Bacillus subtilis |
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| CHEN Sheng,ZHANG Fu-hua,CHEN Jian,WU Jing.Effects of Fed-fermentation on Cutinase Production by Recombinant Bacillus subtilis[J].China Biotechnology,2010,30(1):62-66. |
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| Authors: | CHEN Sheng ZHANG Fu-hua CHEN Jian WU Jing |
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| Institution: | 1.School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China
2.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China |
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| Abstract: | In order to achieve the high production of cutinase by recombinant Bacillus subtilis WSHB06-07,
the effect of various initial sucrose concentration on enzyme production was investigated in 3 L fermentor. It was found that sucrose concentration of 38 g/L is suitable for cell growth and cutinase production. Based on this sucrose concentration, fed-batch as well as constant-fed fermentation were investigated. The results showed that, using constant-fed fermentation from 16 h to 31 h and sucrose feed rate of 4 g/(L· h), the cutinase activity in the culture media reached 545.87U/ml, which was increased by 67.8% compared to that of batch fermentation. By this way, high cutinase productivity was gained and it is beneficial for industrial production. |
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| Keywords: | Bacillus subtilis Recombinant cutinase Fed-batch fermentation Constant-fed fermentation Carbon source Cutinase productivity |
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