Biological and nutritional aspects involved in fern multiplication |
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Authors: | Fernández H Bertrand AM Sánchez-Tamés R |
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Institution: | (1) Lab. Fisiología Vegetal, Dpt. B.O.S. c), Catedrático Rodrigo Uría, s/n Oviedo University, Spain |
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Abstract: | Gametophytes of several species of ferns were mechanically triturated and the resulting homogenates cultured in vitro for
propagation purposes. Differences in the time period from spore culture to sporophyte development were perceivable between
species. For those species with a fast life cycle and high sporophyte production such as Woodwardia virginica and Dryopteris
affinis sp. affinis, homogenization of gametophytes can be considered to be excellent method for propagation, yielding hundreds
of sporophytes in a short period of time. Sporophyte formation was inhibited in O. regalis by the succesive application of
homogenization to gametophytes regenerated by this technique. The effect of the culture medium composition on fern production
was also studied in O. regalis and P. ensiformis gametophytes. In these species, sporophyte formation increased when the gametophytes
were cultured in a medium containing water+0.7% agar. Addition of sucrose inhibited gametophyte development and induced their
necrosis. The 1/2 dilution of Murashige and Skoog basal medium, without sucrose, favoured leaf expansion in P. ensiformis
sporophytes.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Asplenium nidus Dryopteris affinis sp affinis fern Osmunda regalis propagation Pteris ensiformis tissue culture Woodwardia virginica |
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