Evaluation of genetic variation in the daylily (Hemerocallis spp.) using AFLP markers |
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Authors: | J P Tomkins T C Wood L S Barnes A Westman R A Wing |
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Institution: | (1) Clemson University Genomics Institute, Room 100 Jordan Hall, Clemson, SC 29634, USA e-mail: jtmkns@clemson.edu, JO;(2) Department of Biological Sciences, Clemson University, Room 132 Long Hall, Clemson, SC 29634, USA, US;(3) Methodist College, Biology Department, 5400 Ramsey Street, Fayetteville, N.C. 28311, USA, US |
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Abstract: | The daylily (Hemerocallis spp.) is one of the most economically important ornamental plant species in commerce. Interestingly, it is also one of the
most heavily bred crops during the past 60 years. Since the American Hemerocallis Society began acting as the official registry
of daylily cultivars in 1947, more than 40 000 registrations have been processed. In order to determine the effects of intensive
breeding on cultivar development, and to study relationships among different species, genetic variation in the daylily was
estimated using AFLP markers. Nineteen primary genotypes (species and early cultivars) and 100 modern cultivars from different
time periods were evaluated using 152 unambiguous bands (average 79% polymorphism rate) derived from three AFLP primer combinations.
Overall, pairwise similarity estimates between entries ranged between 0.618 and 0.926 (average=0.800). When comparing cultivar
groups from different time periods (1940–1998), genetic similarity was initially increased, compared to the primary diploid
genotypes, remained constant from 1940 to 1980, and then steadily increased as breeding efforts intensified and hybridizers
began focusing on a limited tetraploid germplasm pool derived by colchicine conversion. Among modern (1991–1998) daylily cultivars,
genetic similarity has increased by approximately 10% compared to the primary genotypes. These data were also used to evaluate
recent taxonomic classifications among daylily species which, with a few minor exceptions, were generally supported by the
AFLP data.
Received: 15 March 2000 / Accepted: 13 June 2000 |
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Keywords: | Hemerocallis Daylily Genetic variation AFLP |
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