Molecular Analysis of <Emphasis Type="Italic">Candida albicans</Emphasis> Isolates from Clinical Specimens |
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Authors: | Melahat Gurbuz Ilknur Kaleli |
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Institution: | (1) Department of Microbiology and Clinical Microbiology, School of Medicine, Pamukkale University, Denizli, Turkey;(2) Department of Medical Microbiology, Faculty of Medicine, Pamukkale University, 20100 Denizli, Turkey |
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Abstract: | The aim of this study was to genotype Candida albicans strains isolated from various clinical specimens by using CA-INT-R and CA-INT-L primer pairs designed to span the region
that includes the site of the transposable group-1 intron in the 25S rRNA gene. A total of 194 C. albicans isolates (28 invasive and 166 noninvasive) were genotyped. The frequencies of genotypes A, B, C and D were found as 51.0,
29.4, 19.1 and 0.5%, respectively. Statistically significant difference was determined between frequency of genotype distribution
between invasive and noninvasive isolates (P < 0.001). Genotype C was more prevalent among invasive isolates while genotype A was in noninvasive ones. Furthermore, six
different subtypes were determined among genotype A C. albicans isolates by restriction endonuclease analysis using a previously constructed differentiation scheme consisting of HaeIII and MspI digestions. This study demonstrated the genetic diversity of clinical isolates of C. albicans in our hospital. |
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