Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer by <Emphasis Type="Italic">Azotobacter chroococcum</Emphasis> strain 7B |
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Authors: | V L Myshkina E A Ivanov D A Nikolaeva T K Makhina A P Bonartsev E V Filatova A O Ruzhitsky G A Bonartseva |
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Institution: | (1) Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Yugoslavia;(2) Faculty of Agriculture, University of Novi Sad, 21000 Novi Sad, Yugoslavia |
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Abstract: | The ability of Azotobacter chroococcum strain 7B, producer of poly(3-hydroxybutyrate) (PHB), to synthesize its copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) was studied. It was demonstrated, for the first time, that A. chroococcum strain 7B was able to synthesize P(3HB-co-3HV) with various molar rates of HV in the polymer chain when cultivated on medium with sucrose and carboxylic acids as precursors of HV elements in the PHB
chain, namely, valeric (13.1–21.6 mol %), propanoic (3.1 mol %), and hexanoic (2.1 mol %) acids. Qualitative and functional
differences between PHB and P(3HB-co-3HV) were demonstrated by example of the release kinetic of methyl red from films made
of synthesized polymers. Maximal HV incorporation into the polymer chain (28.8mol %) was recorded when the nutrient medium
was supplemented with 0.1% peptone on the background of 20 mM valerate. These results suggest that that the studied strain
can be regarded as a potential producer of not only PHB but also P(3HB-co-3HV). |
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