| 琼脂糖电内渗对电泳的影响 |
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| 引用本文： | 郭尧君,李辛晖,方勇.琼脂糖电内渗对电泳的影响[J].生物化学与生物物理进展,1997,24(3):268-272. |
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| 作者姓名： | 郭尧君 李辛晖 方勇 |
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| 作者单位： | 中国科学院生物物理研究所，北京 100101;中国科学院生物物理研究所，北京 100101;中国科学院生物物理研究所，北京 100101 |
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| 摘 要： | 用电内渗不同(0、0.03、0.08、0.20mr)的琼脂糖制成凝胶或电极缓冲液凝胶条，观察对电泳行为的影响.结果表明等电聚焦电泳必须使用无电内渗琼脂糖.不同电内渗的琼脂糖制成的电极缓冲液凝胶条对SDS电泳无显著影响，但对常规聚丙烯酰胺凝胶电泳有不同程度的影响，电内渗越高，越不利于电泳的进行.
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| 关 键 词： | 琼脂糖，电内渗，半干电泳，电极缓冲液凝胶条 |
| 收稿时间： | 1996/6/28 0:00:00 |
| 修稿时间： | 1996/11/4 0:00:00 |
The Effects of Electroendosmosis in Agarose on Electrophoresis |
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| GUO Yaojun,LI Xinhui and FANG Yong.The Effects of Electroendosmosis in Agarose on Electrophoresis[J].Progress In Biochemistry and Biophysics,1997,24(3):268-272. |
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| Authors: | GUO Yaojun LI Xinhui and FANG Yong |
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| Institution: | Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | The effects of gel or electrode buffer strips using agarose with different electroendosmosis of 0, 0.03, 0.08, 0.20 mr on electrophoresis were studied. It was shown that only agarose without electroendosmosis can be used for isoelectric focusing. The electrod buffer strips with different electroendosmosis did not affect SDS PAGE significantly but they affect Native PAGE in certain extent. The higher the electroendosmosis was, the more difficultly it ran. |
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| Keywords: | agarose electroendosmosis semidry electrophoresis electrode buffer strips |
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