| 辣根过氧化物酶同工酶在不同介质中的动力学 |
| |
| 引用本文： | 陈文玲,刘峰,林永齐.辣根过氧化物酶同工酶在不同介质中的动力学[J].中国生物化学与分子生物学报,1994,10(1):7-10. |
| |
| 作者姓名： | 陈文玲 刘峰 林永齐 |
| |
| 作者单位： | 吉林大学分子生物学系 |
| |
| 基金项目： | 吉林大学酶工程国家重点实验室资助 |
| |
| 摘 要： | 本文研究了辣根过氧化物酶［ＥＣ１．１１．１．７］同工酶的联苯胺动力学。结果表明：其酸性酶和碱性酶的最适ｐＨ均为５．８左右。二者最适有机溶剂浓度略有差异：酸性酶最适乙醇浓度为５０％，最适二氧六环浓度为４０％；而碱性酶则分别为６０％和５０％。在水溶剂中，酸性酶为米氏酶，碱性酶为正协同的别构酶；在有机溶剂（如：乙醇、二氧六环）中，酸性酶为正协同的别构酶，碱性酶则仍为正协同的别构酶。即有机溶剂可能使酶构象发生变化。
|
| 关 键 词： | 辣根过氧化物酶（酸性酶．碱性酶） 联苯胺 H_2O_2 |
| 收稿时间： | 1994-02-20 |
Kinetics of the Isoenzymes of HRP in Different Media |
| |
| Chen Wen-ling,Liu Feng,Lin Yong-qi.Kinetics of the Isoenzymes of HRP in Different Media[J].Chinese Journal of Biochemistry and Molecular Biology,1994,10(1):7-10. |
| |
| Authors: | Chen Wen-ling Liu Feng Lin Yong-qi |
| |
| Institution: | (Molecular Biology Department, Jilin University, Changchun, 130023 |
| |
| Abstract: | Isoenzymes of Horseradish Peroxidase are studied with benzidine as substrate.The optimal pHs of acid Isoenzyme(A) and basic isoenzyme (B) are all about 5. 8. The optimal concentrations of organic solvents of A and B are slightly different for A in alcohol is 50%,for B in alcohol is 60%.A in dioxane is 40%. B in dioxane is 50%. A reacts as a michaelis-Menten enzyme in H2O, but A reacts as a positive cooperative enzyme in water , alcohol and dioxane. While B is a positive cooperative enzyme in, alcohol and dioxane. It is predicted that alcohol and dioxane can change the conformation of HRP isoenzymes. |
| |
| Keywords: | Horseradish peroxidase(acid enzyme basic enzyme) Benzidine H_2O_2 |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息 |
| 点击此处可从《中国生物化学与分子生物学报》下载免费的PDF全文 |
