| 硒、谷胱甘肽过氧化物酶和不饱和脂肪酸在鼠脑亚细胞中的分布 |
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| 引用本文： | 黄开勋,徐辉碧,王君健.硒、谷胱甘肽过氧化物酶和不饱和脂肪酸在鼠脑亚细胞中的分布[J].中国生物化学与分子生物学报,1995,11(3):264-269. |
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| 作者姓名： | 黄开勋 徐辉碧 王君健 |
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| 作者单位： | 华中理工大学化学系 |
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| 基金项目： | 中国－丹麦科技合作项目,丹麦Danida基金 |
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| 摘 要： | 采用梯度离心和放射性同位素等方法从鼠脑中分离得到髓磷脂、突触囊、轻突触体、重突触体、线粒体６个亚细胞组分。分别测定了各亚细胞中硒－７５、谷胱甘肽过氧化物酶和不饱和脂肪酸的含量，结果表明这些成分在鼠脑亚细胞中的分布呈现明显的相关性，同时首次在突触囊、线粒体和微粒体中检测到三种不同的谷胱甘肽过氧化物酶的活性峰，其中之一可能是红细胞谷胱甘肽过氧化物酶（ＥＣ１．１１．１．９）．还就机体的自我保护机制和硒在脑组织中的重要作用进行了讨论。
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| 关 键 词： | 硒 谷胱甘肽过氧化物酶 不饱和脂肪酸 亚细胞 鼠脑 |
| 收稿时间： | 1995-06-20 |
Subcellular Distribution of Selenium,Glutathione Peroxidases and Unsaturated Fatty Acids in Rat Brain |
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| Huang Kai-Xun,Xu Hui-Bi,Wang Juan-Jian J.Clausen.Subcellular Distribution of Selenium,Glutathione Peroxidases and Unsaturated Fatty Acids in Rat Brain[J].Chinese Journal of Biochemistry and Molecular Biology,1995,11(3):264-269. |
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| Authors: | Huang Kai-Xun Xu Hui-Bi Wang Juan-Jian JClausen |
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| Institution: | (Department of Chemistry,Huazhong University of Science and Technology,Wuhan 430074) (Institute for Life Sciences and Chemistry,Roskilde University,DK-4000 Denmark |
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| Abstract: | By means of gradient ultracentrifugation,the subcellular fractions of the rat brain,myelin(A),synaptic vesicles (B),synaptosomes (C),heavy synaptosomes(D), mitochondria (E) and microsomes (F),were isolated,and the radioactivity of Se-75, glutathione peroxidase (GSH-Px) and unsaturated fatty acids (UFAs) were estimated. The results showed that those components were relative to each other. However, there were three peaks of GSH-Px activity only in fraction B,E,F on gel-filtration of Sephadex G150 (superfine). One of them may be erythrocyte GSH-Px (EC. 1. 1 1. 1. 9) . A system,which inhibits autooxidation of UFAs in rat brain,was discussed. |
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| Keywords: | Selenium Glutathione peroxidase Unsaturated fatty acids Subcellular fraction Rat brain |
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