LIPID COMPOSITION OF GLIAL CELLS ISOLATED FROM BOVINE WHITE MATTER |
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| Authors: | Mona E. Fewster J. F. Mead |
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| Affiliation: | Division of Neurology, Department of Medicine and Departments of Biological Chemistry and of Biophysics and Nuclear Medicine, UCLA School of Medicine, Los Angeles, California |
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| Abstract: | Abstract— —Glial cells were isolated from bovine white matter by differential centrifugation with'Ficoll'and their lipid composition was analysed. The preparations contained 20.8 per cent lipid and 792 per cent protein. The major lipid components were cholesterol, ethanolamine glycerophosphatides (EGP), cerebroside and serine glycerophosphatides (SGP). Sphingomyelin, cerebroside sulphate and inositol glycerophosphatide were present in lower proportions. EGP contained the largest proportion of aldehydes (17 per cent) and SGP contained 12 per cent. Choline glycerophosphatides contained only a trace of aldehyde. No gangliosides were present in the filial cell preparations. |
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