Resistance ofPseudomonas aeruginosa to β-lactam antibiotics |
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Authors: | U L Soto S M Amadou F J Ramos B E Baca |
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Institution: | (1) Departamento de Investigaciones Microbiológicas, Institute de Ciencias, Universidad Autónoma de Puebla, P.O. Box 1622, Puebla, Pue., México |
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Abstract: | A series of experiments were performed withP. aeruginosa to demonstrate which of the biochemical mechanisms are responsible for the resistance to the β-lactam antibiotics. The constitutive
β-lactamase was isolated and characterized for the strain used as type OXA whose pI was 7.1, with a molar mass of 49 kg/mol.
The strain was also submitted to a series of treatments with Tris and Tris -EDTA to disrupt the outer membrane. The treated
cells demonstrated a ten-fold reduction in the MIC with cloxacillin, six-fold with penicillin, and five-fold with oxacillin.
At least two different biochemical mechanisms were responsible for the resistance to the β-lactams studied which could be
important in the prevalence ofP. aeruginosa in nosocomial infections. |
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Keywords: | |
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