Abstract: | The proportion of occluded virions and free virions in infected midgut cells of Malacosoma disstria was determined at advanced stages (10 days) of cytoplasmic-polyhedrosis virus infection. In infected cells, less than 30% of the virions were occluded within polyhedral bodies, and more than 70% were found as free forms. In virus with uridine-3H, it was found that free virions were labeled more quickly than those occluded in polyhedra. Occlusion of virions within the protein matrix of the polyhedral inclusion body did not occur until 5–10 hr after the appearance of free virions. |