小麦耐盐细胞系对盐胁迫的伤害性反应

通过逐级提高ＮａＣｌ浓度的筛选方法，得到了能在１．５％ＮａＣｌ下生长良好的小麦（ＴｒｉｔｉｃｕｍａｅｓｔｉｖｕｍＬ．）耐盐细胞系。在盐分胁迫下，耐盐细胞系含水量的降低幅度小于不耐盐细胞系（对照），Ｈ２Ｏ２含量和Ｏ－２产生速率的增加幅度也明显小于对照细胞系。同时，膜的相对透性、膜脂过氧化和脱酯化程度的提高幅度也明显低于对照细胞系。表明盐分对小麦细胞系膜的伤害与活性氧介导的膜脂过氧化和脱酯化有关，而耐盐细胞系比对照细胞系表现出较强的抗活性氧伤害的能力。

Damage Responses to Salt Stress in Salt tolerant Cell Line of Wheat

A salt tolerant cell line of wheat (Triticum aestivum L.) that could grow well under 1.5% NaCl stress was obtained by gradual increase of NaCl concentration. Under NaCl stress, the water content in the salt tolerant cell line decreased less than that in the salt sensitive cell line served as control, while the H 2O 2 content and O - 2 production rate in the control cell line increased more obviously than those in the salt tolerant cell line. Meanwhile, the membrane relative permeability, membrane lipid peroxidation and deesterification increased more significantly than those in the salt tolerant cell line. These results indicated that the damage of wheat cell lines caused by NaCl stress had close relationship with active oxygen mediated membrane lipid peroxidation and deesterification, and the resistance to the active oxygen mediated damage in the salt tolerant cell line was remarkably higher than that in the control cell line.