| 稻瘟病菌株的DNA指纹分析及小种鉴别 |
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| 引用本文： | 朱衡,蒋琬如,王久林,雷财林,凌忠专,朱立煌.稻瘟病菌株的DNA指纹分析及小种鉴别[J].菌物学报,1992,11(Z1):89-98. |
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| 作者姓名： | 朱衡 蒋琬如 王久林 雷财林 凌忠专 朱立煌 |
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| 作者单位： | 中国科学院遗传研究所;中国农业科学院作物育种栽培研究所;中国农业科学院作物育种栽培研究所;中国农业科学院作物育种栽培研究所;中国农业科学院作物育种栽培研究所;中国科学院遗传研究所 |
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| 摘 要： | 基于对稻瘟病菌(Pyricularia oryzae)基因文库的分析,我们找到了一套含重复顺序的克隆。其中POR6和POR7被证实具有高度的多态性并随机散布于稻瘟病菌生理小种的致病性时,可以获得可分辨的基因组特异的杂交带型。我们还分析了致病性与8个稻瘟病菌株DNA指纹图谱之间的关系,结果表明各个小种组合间的百分相似率Sxy,值与该小种组合间共同侵染的鉴别品种数目有正相关性。
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| 关 键 词： | 稻瘟病菌 DNA指纹分析 探针 分子杂交 |
DNA FINGERPRINTING AND PATHOTYPE CHARACTERIZATION OF PYRICULARIA ORYZAE ISOLATES |
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| Authors: | ZHU Heng JIANG wan-ru WANG Jiu-lin LEI Cai-lin LING zhong-zhuan and ZHU Li-huang |
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| Institution: | Institute of Genetics, Academia Sinica, Beijing;Institute of Crop Breeding and Cultivation, CAAS, Beijing;Institute of Crop Breeding and Cultivation, CAAS, Beijing;Institute of Crop Breeding and Cultivation, CAAS, Beijing;Institute of Crop Breeding and Cultivation, CAAS, Beijing;Institute of Genetics, Academia Sinica, Beijing |
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| Abstract: | Based on an analysis of a genome library of the rice blast fungus, Pyricularia oryzae, a set of clones containing repeated sequences of P. oryzae was identified. Of them, POR6 and POR7 were proved highly polymorphic and random distributed in P. oryzae genomes. When POR6 was used as probe in DNA fingerprinting to investigate pathotypes of rice blast fungus races, distinctive genome-specific profiles were observed. We have analyzed the relationship between the pathotypes and fingerprints of nine blast fungus isolates from Japan and China. The result indicated that the percentage of similar hybridization bands (Sxy) in POR6-DNA fingerprints of any two isolates are positively related to the number of rice cultivars which can be infected by these two isolates. POR6 may become a useful probe in study of diversity and phylogenetic relationship among races of rice blast fungus. |
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| Keywords: | Rice Blast Fungus DNA Fingerprinting Probe Molecular Hybridization |
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