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Immunological detection of bean common mosaic virus in French bean (Phaseolus vulgaris L.) leaves
Authors:Poonam Verma  U. P. Gupta
Affiliation:(1) USDA-ARS, 24106 North Bunn Road, Prosser, WA, 99350, U.S.A.;(2) Novartis Seed Company Inc., Nampa, ID, 83687, U.S.A.;(3) Department of Crop and Soil Sciences, Michigan State University, E. Lansing, MI, 48824, U.S.A.
Abstract:
Bean common mosaic potyvirus (BCMV) is an important seed borne pathogen of French bean. Differential inoculation with bean common mosaic virus at cotylodonary trifoliate leaf stage and pre-flowering stage of crop growth revealed that cotyledonary leaf infection favored maximum disease expression. Under immunosorbent electron microscopy (ISEM) the virus particles of filamentous structure having a diameter of 750 nm (l) and 15 nm (w) were observed. These particles gave positive precipitin tests with polyclonal antiserum of Potato virus Y.
Keywords:Bean common mosaic virus   French bean
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