Ubiquinone and carotene production in the <Emphasis Type="Italic">Mucorales Blakeslea</Emphasis> and <Emphasis Type="Italic">Phycomyces</Emphasis> |
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Authors: | Vera Kuzina Enrique Cerdá-Olmedo |
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Institution: | (1) Departamento de Genética, Facultad de Biología, Apartado 1095, Universidad de Sevilla, E-41080 Seville, Spain;(2) BioCentrum-DTU, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark |
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Abstract: | The filamentous fungi Phycomyces blakesleeanus and Blakeslea trispora (Zygomycota, Mucorales) are actual or potential industrial sources of β-carotene and lycopene. These chemicals and the large terpenoid moiety of
ubiquinone derive from geranylgeranyl pyrophosphate. We measured the ubiquinone and carotene contents of wild-type and genetically
modified strains under various conditions. Light slightly increased the ubiquinone content of Blakeslea and had no effect on that of Phycomyces. Oxidative stress modified ubiquinone production in Phycomyces and carotene production in both fungi. Sexual interaction and mutations in both organisms made the carotene content vary
from traces to 23 mg/g dry mass, while the ubiquinone content remained unchanged at 0.3 mg/g dry mass. We concluded that the
biosyntheses of ubiquinone and carotene are not coregulated. The specific regulation for carotene biosynthesis does not affect
even indirectly the production of ubiquinone, as would be expected if terpenoids were synthesized through a branched pathway
that could divert precursor flows from one branch to another. |
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Keywords: | |
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