Evaluation of membrane filter methods for DNA-DNA hybridization |
| |
Authors: | J. De Ley R. Tijtgat |
| |
Affiliation: | (1) Laboratory for Microbiology and Microbial Genetics, Faculty of Sciences, State University, Gent, Belgium |
| |
Abstract: | Three membrane filter methods for DNA hybridization were compared. In all of them filter-fixed DNA is spontaneously released during incubation. The effect depends largely on the temperature: 40 to 85% of DNA is released at 70 to 80 C; at higher temperatures most of the DNA is released and heterologous DNA relatedness values are unreliable. Only the dimethylsulfoxide filter method (Legault-Démare et al., 1967) can be recommended for the entire temperature and % GC range. The methods of Denhardt (1966) and of Warnaar and Cohen (1966) are not recommended for hybridizations of DNA containing more than 50% GC. Impure DNA yields too low DNA relatedness values. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|