Some factors affecting root formation onin vitro regenerated pea shoots |
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Authors: | Marie Kubaláková Eva Tejklová M Griga |
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Institution: | (1) Institute of Experimental Botany, Czechoslovak Academy of Sciences, CS-772 00 Sokolovská 6, Olomouc, Czechoslovakia;(2) OSEVA Research and Breeding Institute of Technical Crops and Legumes, CS-787 01 Šumperk-Temenice, Czechoslovakia |
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Abstract: | The rooting ability of 2 cm long shoots ofPisum sativum L., derived from differentin vitro shoot-tip cultures in two pea cultivars Bohatýr and Kleine Rheinländerin was evaluated. In three mutually independent experiments the full and half-strength Murashige-Skoog medium (containing full or half concentration of macro and microelements), with sucrose concentrations 10–30 g l-1, and with various NAA and IAA combinations, was tested. The variant with half concentration of macro- and microelements, supplemented with 30 g l1 sucrose, and with growth regulators in the quantity of 1 μM proved optimum. |
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