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| 幽门螺杆菌临床分离株的随机扩增多态性DNA指纹图分析 | |
| 引用本文： | 许迪,叶嗣颖,孔利佳,彭颖,黄庆华,廖方.幽门螺杆菌临床分离株的随机扩增多态性DNA指纹图分析[J].中国微生态学杂志,2004,16(1):5-7. |
| 作者姓名： | 许迪 叶嗣颖 孔利佳 彭颖 黄庆华 廖方 |
| 作者单位： | 1. 华中科技大学同济医学院,实验动物学部,湖北,武汉,430030 2. 华中科技大学同济医学院,基础医学院微生物学教研室,湖北,武汉,430030 3. 温州医学院,细胞与分子医学研究所,浙江,温州,325027 |
| 摘 要： | 目的:建立武汉及周边地区幽门螺杆菌(Helicobacter pylori,Hp)感染病人胃内分离的Hp的DNA指纹图谱,并进行数理统计分析,探讨Hp基因型与疾病的相互关系,为临床诊断、防治及致病机制提供理论与实践基础.方法:采取随机扩增多态性DNA指纹法(Random amplified polymorphic DNA,RAPD)对48例病人Hp基因组DNA进行PCR反应,其随机引物为:1290 5'-GTGGATGCGA-3'.反应产物经2%琼脂糖凝胶电泳,成像存盘.用统计分析软件(Statistic analysis software,SAS)对Hp DNA指纹图的相似性以及与疾病的相关性进行分析.结果:每个菌株都有其独特的DNA指纹图,显示其基因的多态性;计算机聚类分析显示:Hp DNA指纹图可分为两大类,其与宿主疾病之间有一定程度的相关性(P＜0.05).结论:(1)RAPD对 Hp DNA扩增结果是稳定、可靠的,是一种较好的分型方法.(2)幽门螺杆菌感染所致疾病可能与其基因型相关. |
| 关 键 词： | 螺杆菌 幽门 随机扩增多态性DNA 聚类分析 |
| 文章编号： | 1005-376X(2004)01-0005-03 |
| 修稿时间： | 2003年8月20日 |
Analysis of the random amplified polymorphic DNA fingerprinting of Helicobacter pylori clinical strains |
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| XU Di,YE Si ying,KONG Li jia,et al.Analysis of the random amplified polymorphic DNA fingerprinting of Helicobacter pylori clinical strains[J].Chinese Journal of Microecology,2004,16(1):5-7. | |
| Authors: | XU Di YE Si ying KONG Li jia |
| Abstract: | Objective:To establish the random amplified polymorphic DNA (RAPD) fingerprinting of Helicobacter pylori isolates from Wuhan city and nearby areas ,and to investigate the relationship between RAPD genotyping and pathogenicity of Helicobacter pylori Methods:Using RAPD to genotype the 19 Hp strains isolated from gastritis patients and 29 strains from peptic ulcer ones,and using SAS software to do cluster analysis.Results:(1)RAPD fingerprinting can detect the genetic diversity of Hp;(2)According to their genotype profiles,Hp strains are divided into two category in which the rate of strains of the two sources shows apparent difference( P <0 05) Conclusion:(1)RAPD analysis is a better genotyping system;(2)There maybe exist some genotypes related to pathogenicity of Hp. |
| Keywords: | Helicobacter pylori RAPD Cluster analysis |
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