A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp. |
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| Authors: | A G Schijman M E Floccari B S Méndez |
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| Institution: | (1) Departamento de Química Biológica. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina |
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| Abstract: | Summary A rapid method was developed for plasmid DNA screening from micro volume cultures of Clostridium acetobutylicum. It involves protoplastization by lysozyme, nuclease inhibition by incorporating EDTA or DEP, followed by lysis with high concentration of SDS. The whole lysate is applied directly to electrophoretic analysis. |
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