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Measurement of Serine Acetyltransferase Activity in Crude Plant Extracts by a Coupled Assay System Using Cysteine Synthase |
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| Authors: | Nakamura Katsuhito; Hayama Atsushi; Masada Masahiro; Fukushima Kazuo; Tamura Goro |
| Institution: | 1Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University Matsudo, Chiba 271, Japan 2Department of Biology, Faculty of Science, Tokyo Metropolitan University Setagaya, Tokyo 158, Japan 3Department of Microbiology, Nihon University School of Dentistry at Matsudo Matsudo, Chiba 271, Japan |
| Abstract: | Serine acetyltransferase (SATase) (EC 2.3.1.30 EC] ) catalyzes theformation of Oacetyl-L-serine (OAS) from L-serine in the presenceof acetyl-CoA. A novel assay method was developed for measuringthis enzyme activity in extracts from plant tissues. The assayconsists of a coupled system in which the OAS formed is convertedto cysteine by the addition of cysteine synthase (CSase) (EC4.2.99.8 EC] ). Cysteine thus formed is determined colorimetricallyand serves as a measure for SATase activity. This method israpid, simple and sensitive, and can be readily adapted formeasurement of SATase activity in crude tissue extracts or homogenates. (Received January 14, 1987; Accepted April 27, 1987) |
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