猪H-FABP基因多态片段的序列分析

利用PCR-RFLP方法在猪H-FABP基因内确定了3个变异酶切位点，分别为5′-上游区HinfI-RFLP、内含子2的HaeIII-RFLP和HinfI*-RFLP（HinfI和HinfI*代表不同区域的HinfI酶切反应）。对每个多态片段进行克隆测序分析，结果表明,5′-上游区HinfI-RFLP是由于1324位的碱基T→C的突变引起；在内含子2中，HaeIII-RFLP变异酶切位点在1811位，发生了C到G的突变；HinfI*-RFLP是由于1970位发生T→C的碱基替换。 Abstract:Three variant restriction sites of porcine H-FABP gene,including HinfI-RFLP in 5′-upsream,HaeIII-RFLP and HinfI*-RFLP in intron 2,were confirmed by PCR-RFLP method.The polymorphic fragments were cloned and sequenced.The results revealed a single nucleotide substitution of T→C at position 1324 for HinfI-RFLP,C→G at position 1811 for HaeIII-RFLP and T→C at position 1970 for HinfI*-RFLP,respectively.

Sequence Analysis of Polymorphic Fragments of Porcine H-FABP Gene

Three variant restriction sites of porcine H-FABP gene,including HinfI-RFLP in 5'-upstream, HaeIII-RFLP and HinfI*-RFLP in intron 2, were confirmed by PCR-RFLP method. The polymorphic fragments were cloned and sequenced. The results revealed a single nucleotide substitution of T-->C at position 1324 for HinfI-RFLP,C-->G at position 1811 for HaeIII-RFLP and T-->C at position 1970 for HinfI*-RFLP, respectively.