Expression of Escherichia coli ribosomal protein and RNA polymerase genes cloned on plasmids. |
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| Authors: | N. P. Fiil D. Bendiak J. Collins J. D. Friesen |
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| Affiliation: | (1) University Institute of Microbiology, DK-1353 Copenhagen K, Denmark;(2) Department of Biology, York University, M3J 1P3 Downsview, Ontario, Canada;(3) Abteilung Genetik, Gesellschaft für Biotechnologische Forschung abH, D-3300 Braunschweig-Stockheim, Federal Republic of Germany;(4) Present address: Department of Microbiology & Molecular Genetics, Harvard University School of Medicine, 25 Shattuck Street, 02115 Boston, MA, USA |
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| Abstract: | ![]()
Summary Fragments of  drifd 18 DNA with different end-points within the set of structural genes of ribosomal proteins L11 (rplK), L1 (rplA), L10 (rplJ) and L12 (rplL) as well as the  (rpoB) and  (rpoC) subunits of RNA polymerase have been cloned on plasmids. These plasmids were transformed in host cells which were mutant for each of the genes, enabling expression of both wild-type (plasmid-borne) and mutant (chromosomal) genes to be differentiated. On the basis of these results we propose the following genetic structure for the region: rplK and rplA are in one operon; rplL, rpoB and rpoC are in a second. Our data suggest the possibility that rplJ is by itself in an operon situated between the other two. |
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