江西省2010年柯萨奇病毒A组24型变异株的全基因组序列分析

急性出血性结膜炎(Acute hemorrhagic conjunctivitis,AHC)是目前人类最常见的眼病之一,柯萨奇病毒A组24型变异株(Coxsackievirus A24 variant,CV-A24v)是近年来报道引起该病的主要病原体。本研究选取10株来自江西省2010年AHC暴发疫情的CV-A24v,采用特异性引物扩增并测定其全基因组序列。对该10条CV-A24v的全基因组序列进行系统发育分析以及重组分析,计算本研究测定的江西10条以及GenBank中所有22条CV-A24v的全基因组序列的氨基酸置换熵值,并预测其正向选择位点。结果表明,在江西10条CV-A24v基因组序列中未检测到重组。基于全基因组序列构建的最大似然树表明江西10株CV-A24v属于GIV基因型,且分处于两条传播链。对上述32条CV-A24v序列的氨基酸置换熵值计算,共得到25个易突变位点(熵值>0.6),易突变概率最高的区段为2A区。基于Datamonkey中FUBAR和FEL模型分析,发现位于结构蛋白VP2区的234位氨基酸为两种模型共同获得的CV-A24v的正向选择位点。本研究分析了江西10株CV-A24v的全基因组序列特征,为CV-A24v引起的AHC防控工作提供了基础资料。

Whole Genome Sequence Analysis of Coxsackievirus A24 Variant Isolated in Jiangxi Province,China,2010

Acute hemorrhagic conjunctivitis(AHC)is one of the most common eye diseases in humans.Coxsackievirus A24 variant(CV-A24 v)is the main pathogen of AHC reported in recent years.We selected 10 strains of CV-A24 v isolated from the 2010 AHC outbreak in Jiangxi Province,China to identify their whole genome sequences.Phylogenetic and recombination analyses based on the whole genome sequences of the 10 strains of CV-A24 v from Jiangxi Province,China were conducted.The amino acid replacement entropy of the whole genome sequences of the 10 strains of CV-A24 v from Jiangxi Province,China and all 22 strains of CVA24 v from GenBank were calculated and their positive selection sites were predicted.Recombination was not detected in the 10 strains of CV-A24 v from Jiangxi Province,China.The maximum likelihood phylogenetic tree based on the whole genome sequence showed that the 10 strains of CV-A24 v from Jiangxi Province,China belonged to GIV,and were divided into two chains of transmission.Amino acid replacement entropy showed that CV-A24 v contained 25 prone mutation sites(entropy>0.6),among which the mutation probability of the non-structural protein 2 A region was the highest.Datamonkey analysis showed that the 234 th amino acid located in the VP2 region was the positive selection site of CV-A24 v obtained jointly based on FEL and FUBAR models.We analyzed the genomic sequence characteristics of 10 strains of CV-A24 v from Jiangxi Province,China.In this way,we provided basic data for control and prevention of AHC caused by CV-A24 v.