PTEN、HPV16/18-E6蛋白和雌激素受体在宫颈腺癌中的表达及其临床意义

目的：探讨宫颈腺癌组织中抑癌蛋白PTEN、人乳头瘤病毒（HPV）16/18-E6蛋白和雌激素受体（ER）的表达及其临床意义。方法：采用免疫组织化学S-P法对65例宫颈腺癌组织进行PTEN、HPV16/18-E6蛋白和ER检测,对30例慢性宫颈炎组织中HPV16/18-E6蛋白和ER的表达进行检测。结果：宫颈腺癌组织细胞核中PTEN的表达显著低于癌旁宫颈腺上皮组织（P〈0.01）,96%的宫颈腺癌细胞核呈低表达,而仅35%的癌旁宫颈腺上皮呈低表达（P〈0.01）;HPV16/18-E6蛋白和ER在宫颈腺癌中的阳性表达率分别为32.1%与49.4%,均显著高于慢性宫颈炎组织（P〈0.01）;HPV16/18-E6蛋白和ER的表达与宫颈腺癌的病理学分级、临床分期无关,在宫颈腺癌中的表达呈正相关。结论：PTEN在宫颈腺癌的发生中起一定的作用,其抑癌作用环节可能在细胞核水平;部分宫颈腺癌的发病可能与HPV16/18-E6蛋白过度表达有关;部分宫颈腺癌可能属于激素依赖性,雌激素可能有协同人乳头瘤病毒致癌的作用。

Expression of PTEN, HPV16/18-E6 and Estrogen Receptor and its Clinical Significance in Uterine Cervical Adenocarcinoma

Objective： To study the expression of PTEN, human papillomavirus（HPV）16/18-E6 and estrogen receptor（ER） and its clinical significance in uterine cervical adenocarcinoma. Methods： The expression of PTEN, HPV16/18-E6 and ER was detected by immunohistochemical S-P method in 65 cases of cervinoma and the same method for the expression of HPVI6/18-E6 and ER in 30 cases of cervical chronic inflammation. Results： In the nucleus, expression of PTEN in the carcinoma was significantly reduced versus normal epithelium （P〈0.01）, 96% of tumors were reduced while only 35% normal were reduced （P〈0.01）. The positive rates of HPV16/18-E6 and ER in cervical adenocareinoma were 52.1% and 49.4%, respectively, significantly higher than that in cervical chronic inflammation（P〈0.01）. Conclusion： PTEN may exert tumor suppression in the nucleus level and involved in the carcinogenesis of adenoeareinoma of cervix. The over expres- sion of HPV16/18-E6 may play an important role in the pathogenesis of cervical adenocareinoma. This result indicates that some of the cervical adenocarcinoma are estrogen dependent, and the estrogen may participate in the onset and progression of the tumor.