A rapid method for the purification of RNA polymerase holoenzyme from Escherichia coli |
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| Authors: | M Mehrpouyan W S Champney |
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| Affiliation: | Department of Biochemistry, College of Medicine, East Tennessee State University, Johnson City 37614. |
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| Abstract: | ![]()
A method is described for the rapid purification of RNA polymerase holoenzyme from small amounts of Escherichia coli cells. Chromatography of a crude extract on a single-stranded DNA agarose column followed by gel filtration chromatography gave 95% pure holoenzyme. The enzyme had kinetic characteristics on T7 DNA identical to those of RNA polymerase purified by other more laborious procedures. |
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