A lectin from Bryonia dioica root stocks |
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Authors: | Willy J Peumans M Nsimba-Lubaki Albert R Carlier Edilbert Van Driessche |
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Institution: | (1) Laboratorium voor Plantenbiochemie, Katholieke Universiteit, Kardinaal Mercierlaan 92, B-3030 I euven (Heverlee);(2) Laboratorium voor Chemie der Proteïnen, Instituut voor Molekulaire Biologie, VUB, Paardenstraat 65, B-1640 Sint-Genesius-Rode, Belgium |
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Abstract: | An N-acetylgalactosamine-specific lectin has been isolated from root stocks of Bryonia dioica by affinity chromatography on fetuin-agarose. It is a dimeric protein composed of two different subunits of relative molecular masses 32,000 and 30,000, held together by intermolecular disulphide bonds. Although most abundant in root stocks, the lectin occurs in all vegetative parts of the plant but not in seeds. Bryony lectin differs from other Cucurbitaceae lectins and from all known N-acetylgalactosamine-specific lectins.Abbreviations BDA
Bryonia dioica agglutinin
- Mr
relative molecular mass
- PBS
phosphate-buffered saline
- SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis |
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Keywords: | Bryonia Lectin N-acetylgalactosamine |
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