Plant regeneration by organogenesis in Vitis rootstock species |
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Authors: | Elisabeth Clog Paul Bass Bernard Walter |
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Institution: | (1) INRA, Station de Recherche Vigne et Vin, Laboratoire de Pathologie Végétale, F-68021 Colmar, France |
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Abstract: | A procedure for the regeneration of Vitis rootstocks plantlets by organogenesis from foliar tissues is described. Leaves from mature plants grown in growth chambers or from plantlets grown in tubes were wounded with a scalpel and cultured on a modified Murashige and Skoog liquid medium containing different concentrations of benzyl-aminopurine. The presence of benzyl-aminopurine is required for shoot formation. The age of the source explant, the composition of the culture medium and the culture temperature are important parameters of the regeneration process.Abbreviations BA
6-benzyl-aminopurine
- MS
Murashige and Skoog medium
- MM
modified Murashige and Skoog medium |
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