Isolation of Chinese hamster ovary cell lines producing Man3GlcNAc2 asparagine-linked glycans. |
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| Authors: | Y Zeng M A Lehrman |
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| Affiliation: | Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235. |
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| Abstract: | Chinese hamster ovary lines with two mutations, one causing accumulation of Man5GlcNAc2-P-P-dolichol and a second resulting in defective N-acetylglucosaminyltransferase I activity, synthesize asparagine-linked glycans with the structure Man3GlcNAc2. As a result, the asparagine-linked glycans produced by these lines are smaller and less heterogeneous than those produced by other currently available animal cell lines. |
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